Genetic diversity among cardamom germplasm accessions assessed by SSR and ISSR analysis

Abstract: Cardamom (Elettaria cardamom Maton; Zingiberacea), the ‘Queen of spices’ is one of the most important and highly priced spices. The important areas of cultivation in Kerala are Nelliampathy, Wynad and Idukki. The origin of cardamom is believed to be in the Western Ghats, where presumably rich and untapped genetic diversity of the cardamom still resides. Biodiversity present in the wild relatives of crop plants in Kerala have not yet been addressed properly (Nayar, 2011). The present study aims to collect and evaluate genetic diversity among wild cardamom populations in comparison to the cultivated counterparts.

Citation

Ashitha J P, Anija T A, Anjali, N and K K Sabu. 2013. Genetic diversity among cardamom germplasm accessions assessed by SSR and ISSR analysis. Proc of the 25th Kerala Science Congress held at Technopark at Thiruvananthapuram during 29th January to 1st February 2013, pp. 263-265 (03-27).

Link to Google Scholar citation

Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

Genet. Mol. Res. 11 (3): 3277 - 3289

DOI: 10.4238/2012.September.12.11

Authors: H.A. Rahim, M.A.R. Bhuiyan, L.S. Lim, K.K. Sabu, A. Saad, M. Azhar, R. Wickneswari
Abstract:
Advanced backcross families derived from Oryza sativa cv MR219/O. rufipogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC2F3 families. Another linkage map was also constructed based on 31 BC2F5 families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufipogon against M. oryzae pathotypes.

Read or download Full Text