The development of angiogenesis plays an essential role in the growth and survival of endometriosis. The aim was to determine the in vitro anti-angiogenic effect of Saraca asoca, Glycyrrhiza glabra, and Curcuma longa ethanolic extract in chick embryos by emphasizing its total phenolic and flavonoid contents in the sample. It was determined by Folin-Ciocalteu and aluminum chloride methods respectively. Anti-angiogenesis assay was done in chorioallantoic membrane. The high amount of total phenol and flavonoid was obtained from all three plant extracts and may be responsible for the bioactivity of the crude extract. Saraca asoca exhibits high phenolic content and anti-angiogenic activity followed by Curcuma longa with rich flavonoid and Glycyrrhiza glabra showed moderate activity for all the assays. A significant anti-angiogenic effect was observed for Saraca asoca (73.65+0.021) % and Curcuma longa (67.65+0.024) % than reference drug dienogest (62.42+0.02) % in the study. Search for new therapeutic agents with anti-angiogenic potential rich in plant secondary metabolites like phenols and flavonoids are need of the hour. The results successfully reveal that the abovementioned plants used in traditional medicines could be used for treating angiogenesis-related diseases like endometriosis.
Thursday, April 18, 2024
Wednesday, August 2, 2023
Phytochemical Profiling and Biological Effects of Glycyrrhiza glabra L.
Glycyrrhiza glabra L. commonly known as licorice belonging to the family Leguminosae is an ayurvedic medicinal plant having substantial pharmacological properties such as anti-inflammatory and anticancer effects. Analysed ethnopharmacological studies show phytochemical and biological activities of the plant extract and serve as a resource for studying anti- endometriosis. According to WHO, 176 million women in the world are affected with endometriosis condition and up to 30-50% of women may experience infertility. Our study aims to evaluate in vitro activity of ethanolic extract of Glycyrrhiza glabra in different cell lines, DPPH assay and HPLC studies in order to propose it to be an effective remedy in endometriosis treatment. In vitro cytotoxic assessment of the extract was determined in estrogenic (MCF 7), non-estrogenic (MDAMB 231) and endometrial cancer cell lines (Ishikawa) along with its safety assessment in normal cell lines (L929). The extract of G. glabra exhibited DPPH radical scavenging activity with 77.09% inhibition in 100 μg concentration. Cytotoxic activity of extract in MCF 7, MDAMB 231, endometrial cancer cells- Ishikawa and significant cell viability in normal L929 cell line shows promising effect of the extract and suggests it as a possible therapeutic adjuvant for treating endometriosis.
